Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia.
نویسندگان
چکیده
The ability of human spermatozoa to exhibit sperm-oocyte fusion in response to the ionophore, A23187, was examined in relation to the capacity of these cells to generate reactive oxygen species. In 70 fertile control donors, there was an overwhelming pattern of high levels of sperm-oocyte fusion associated with low levels of reactive oxygen species production. By contrast, 88% of the 74 oligozoospermic patients exhibited less than 25% oocyte penetration in response to A23187 and 58% exhibited no penetration whatsoever. Of the 40 oligozoospermic patients who failed to respond to A23187, nine had low levels of reactive oxygen species production in association with impaired liquefaction of seminal plasma. Of the remainder, 17 (55%) exhibited defective sperm function together with elevated production of reactive oxygen species. These observations, which are the first to describe a biochemical defect in the spermatozoa of oligozoospermic patients, may carry significant implications for the etiology and treatment of this condition.
منابع مشابه
Impact of Reactive Oxygen Species on Spermatozoa: ABalancing Act between Beneficial and Detrimental Effects
Reactive oxygen species (ROS)plays an important role in sperm motility. The physiological generation at low concentration induces beneficial effects on sperm functions and plays a significant role in sperm metabolism. Meanwhile, the excessive generation of reactive oxygen species can overwhelm protective mechanism and triggers changes in lipid and protein layers of sperm plasma membrane, which ...
متن کاملReactive Oxygen Species and Antioxidant in Seminal Plasma and Their Impact on Male Fertility
Spermatozoa generate reactive oxygen species (ROS) in physiological amounts which play a role in sperm functions during sperm capacitation acrosome reaction (AR) and oocyte fusion. In addition damaged sperm are likely to be the source of ROS. The most important ROS produced by human sperm are hydrogen peroxide superoxide anion and hydroxyl radicals. Besides human seminal plasma and sperm posses...
متن کاملP-87: Effect of Oxidative Stress on Sperm quality and Testis Histopathology in Mature Male Mice
Background: Infertility is a major clinical and worldwide reproductive problem that affects approximately 15% of young couples. The male factors are responsible for periphery 50 percent of these cases. Recently, the generation of oxidative stress, also described as an imbalance between the production of Reactive oxygen species (ROS) and the body’s antioxidant defence mechanisms, in the male rep...
متن کاملI-15: Effects of Oxidative Stress on Sperm Function
Semen analysis is the frontline and old reliable test in the assessment of male fertility, but it failed to provide absolute prediction of sperm function and its fertilizing ability. A wide variety of internal and external factors affect sperm function without discernible and significant effects on semen analysis. Oxygen toxicity and oxidative stress (OS) are inherent challenge of living cells ...
متن کاملEffect of vitamin D on apoptotic marker, reactive oxygen species and human sperm parameters during the process of cryopreservation
Objective(s): Sperm cryopreservation plays an undeniable role in assisted reproductive technology. However, this process significantly reduces the motility, viability, morphology and nuclear integrity of sperm. Reasons of these changes were oxidative stress and apoptosis. The aim of this study was to evaluate the influence of vitamin D on the survival and integrity of ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of andrology
دوره 10 3 شماره
صفحات -
تاریخ انتشار 1989